The fermentation tank then directly enters the steam from the air inlet, discharge port and sampling port into the tank (if there is a sight tank, the steam is also fed together), so that the tank temperature rises to 118-120 ℃, the tank pressure is maintained at 0.09-0.1mpa (gauge pressure), and maintained for about 30min. After the completion of heat preservation, close the exhaust and inlet valves one by one. When the pressure in the tank is less than the air pressure, enter sterile air into the tank, cool down the temperature of the culture medium by cooling water in the jacket or snake tube, reduce the temperature of the culture medium to the required temperature, and carry out the next stage of fermentation and culture.

Fermentation tank

1. Attention to pressure: during sterilization, the pressure standard of main steam pipeline shall not be less than 0.3 ~ 0.35Mpa, and the application pressure shall not be less than 0.2MPa.) After sterilization: when the temperature is 30 ℃ after sterilization in the consumption of glycerine stage: when the temperature is 30 ℃, start stirring (higher rotation speed) and ventilation (0.1-1.0vvm), connect 28% ammonia water (undiluted) to adjust pH5.0; add 4.35ml of sterile ptm1 base salt per liter; inoculate the seed liquid from the shaker, the do value is, which will be consumed at the beginning of culture, resulting in a decrease of do value, pass oxygen to ensure that the do value exceeds 20%, and the rate is 0.1vvm first.